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OXP-06 Refractometer

  Topics:
Index of Refraction
Prism
Total Reflection
Dispersion
Shadow Line
 

Ernst Abbé
1840 - 1905

 


Ernst Abbé was not the first and not the only scientist who developed a convenient refractometer, but his refractometer is – in several varied and updated versions – the most widespread and most common refractometer nowadays. After the first half of the 20th century, modern spectroscopic methods have replaced the refractometer as an analytical instrument. Nevertheless, it is still an essential instrument for empirical identification of pure substances, purity measurements, and quantitative analysis of solutions.
Today the refractometer is mainly used in the food and beverage industry as well as in the chemical and pharmaceutical industry for quality control and check-ups on compound identities. Thus the refractometer developed from an analytical instrument in basic research to a tool for routine measurements in industry.
Several types of refractometers are discussed and their principle of operation is explained. Experiments are performed on a model of an Abbé refractometer to show the implementation on modern, computer driven standard refractometers. This refractometer measures refraction of liquids, solutions and solid materials by observing the shadow line of the light beam on a screen.

 


 

Examples of Investigation and Measurement


Calibration and measurement of several
liquids

With liquids of a known refraction indices the apparatus will be calibrated. Therefore the shadow line of the beam is observed on a screen with a scale. Liquids like water, acetone or glycerol are used for a first measurement. The values of the indices are compared to values found in literature and an estimation of the reliability can be done.

Measurement of solid materials
In the same way refraction indices of solid materials like glasses and crystals can be determined. For these measurements the material should have at least one plain and polished surface.

Measurement of solved sugar solutions
First measurements on sugar solutions with different concentrations of known values will be performed. In the next step the refraction index of a solution with an unknown concentration is measured. Through comparison with the series of measurements the concentration can be deduced.

 


 

 

 

 

OXP-06   Required Equipment


1  
 


02.0500
 

 


Profile rail OCM 650, 500 mm

The high precision optical rails are made out of special anodized aluminum. The rails are the base for various modules attached to carriers.


 




 
1 02.1022 Carrier OCM 650, 20 mm with screen holder

This module can mount optical screens. Through the attached carrier, the screen holder can be placed onto the optical rail. The carrier 20 mm and the holder are made out of special anodized aluminum.
 
1 02.1606 Screen with vertical scale

Experimental results like colour spectra and interference patterns can be visualized on these screens. Horizontal or vertical scales allow calibrations and quantitative measurements; a screen with an aperture is used for observing back-reflected rays. The screens are made out of anodized aluminum plates with one side painted white and can be fixed onto screen holders (02.1022, 02.1608).
 

2

02.2132

Mounting plate OCM 650 for click 30, including carrier 20 mm

Mounting plates are used to hold optical mounts. A characteristic feature of the mounting plates is the “click” mechanism of the inserts based on spring loaded spheres. Snapping in the groove of the inserted click mount, the optical element is kept in an exact position. On the other hand, the system allows a quick and easy change of the mounted inserts.
The mounting plates are made out of special anodized aluminum. Mounted onto the carrier 20 mm, the mounting plates can be placed onto an optical rail.


 

1

02.6106

4 axis adjustment holder KH650, theta, phi, X and Y, including carrier 20 mm

This adjustment holder mounts a Diode Laser or an LED lamp. Using the fine pitch adjustment screws, the optical axis can be aligned within the range of 3 mm. For fine alignment of the angle of the light beam, two additional pitch adjustment screws are attached onto the back of the holder. The holder and the carrier 20 mm are made out of special anodized aluminum and can be placed onto an optical rail.


 

1 04.0051
04.0060

Biconvex lens f=100 mm, mounted in click 30 mount
Plano convex lens f=40, mounted in click 30 mount

Different glass lenses are mounted onto a special anodized aluminum click mount 25 mm by two threaded mounting rings to be used in connection with a mounting plate (02.2126).
 

1 04.0108 Polarizer in click 30 mount

A polarizer high quality plastic foil is mounted onto a special anodized aluminum click mount 30 mm by two threaded mounting rings to be used in connection with a mounting plate (02.2132). The unit is used to polarize the light of an unpolarized light source and can be directly attached to the optical rails.
 

1 05.0034 LED yellow in housing

As universal and easy-to-change light sources, LED lamps are used in optical experiments. The LEDs are mounted onto a 40 mm x 25 mm diameter housing tube made out of special anodized aluminum. For the alignment, the lamps can be fixed onto a four axis adjustment holder mounted onto a carrier. For their operation, the active LDD-05 power supply (07.0206) is required which controls the output power. For the lamps 1 W (white LED: 3W), high brightness LEDs are used.
 

1

07.0206

LDD-05 active power supply

The universal LDD-05 active power supply is used for all laser and LED light sources. It recognizes which source is connected and sets the parameters for it automatically. The unit is equipped with a main switch, a regulation knob for adjusting the laser or LED power and a safety lock. The provided USB bus interface allows control through a personal computer or laptop and qualifies this unit as multimedia source.
The housing is made out of shock-proof plastic with an aluminum front and rear panel.

Voltage: 230 VAC / 50 Hz
Case dimensions: 200 mm x 160 mm x 62 mm.

 






 
1 09.1620 Dove prism assembly

This unit consists of a modern realization of a refractometer arrangement using a dove prism as the optical element. For cleaning purposes, the prism can be easily removed. The unit is mounted onto a holder with carrier 65 mm and can be directly attached to the optical rail. The metal parts are made out of special anodized aluminum. The dove prism is made out of the high refractive index glass SF10.

Dimensions: 26 mm height, 20 mm thickness
Window area for measurements: 20 mm x 20 mm
Base angle: 60°.
 






 


 


 




 

 

Experiments

OXP-01 Refraction of Light

OXP-02 Prisms

OXP-03 Lenses

OXP-04 Reflection & Transmission

OXP-05 Beam Bending

OXP-06 Refractometer

OXP-07 Diffraction of Light

OXP-08 Optical Gratings

OXP-09 Spectral Analysis

OXP-10 Interference

OXP-11 Interferometer

OXP-12 Holography

OXP-13 Polarisation

OXP-14 Double Refraction

OXP-15 Colour Mixing

OXP-16 Optical Filters

OXP-17 Absorption & Emission

OXP-18 Image Projection

OXP-19 Camera

OXP-20 LED & Laser Diode

 

Kits

Basic Kit

Complete Kit

Advanced Kit